| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bradley, Addison O (12)
|
MRLBA |
36.40 |
36.55 |
0.15 |
| 2 |
Brink, Keelyn R (11)
|
MRWISC |
39.15 |
37.64 |
-1.51 |
| 3 |
Singer, Keira A (11)
|
MRWISC |
43.13 |
42.85 |
-0.28 |
| 4 |
Benavides, Genesis S (12)
|
MRWISC |
43.04 |
42.98 |
-0.06 |
| 5 |
Miller, Caroline A (11)
|
MRLBA |
43.98 |
43.74 |
-0.24 |
| 6 |
Mallia, Sarah F (11)
|
MRLBA |
44.01 |
45.12 |
1.11 |
| 7 |
Policaro, Ava N (11)
|
MRLBA |
45.59 |
45.22 |
-0.37 |
| 8 |
Mozian, Alexis P (12)
|
MRSSC |
48.18 |
45.57 |
-2.61 |
| 9 |
Schrader, Eva K (12)
|
MRSSC |
47.24 |
46.84 |
-0.40 |
| 10 |
Salmi, Zaineb (11)
|
MRLIE |
1:00.00 |
46.88 |
-13.12 |
| 11 |
O'Connell, Maggie M (12)
|
MRTOBS |
47.72 |
47.02 |
-0.70 |
| 12 |
Young, Sydney B (12)
|
MRSSC |
47.31 |
47.66 |
0.35 |
| 13 |
Marotta, Alexa P (12)
|
MRSSC |
47.82 |
48.02 |
0.20 |
| 14 |
Gramarossa, Angelina A (12)
|
MRSSC |
51.33 |
48.82 |
-2.51 |
| 15 |
Bannister, Samantha A (11)
|
MRLBA |
48.56 |
49.22 |
0.66 |
| 16 |
Santagata, Victoria E (11)
|
MRSSC |
52.27 |
49.28 |
-2.99 |
| 17 |
Matos, Elisa M (11)
|
MRLBA |
52.44 |
50.28 |
-2.16 |
| 18 |
Simo, Gianna L (11)
|
MRWISC |
52.79 |
50.99 |
-1.80 |
| 19 |
Brill, Kacey (11)
|
MRLBA |
|
51.12 |
- |
| 20 |
Campbell, Taylor M (11)
|
MRLBA |
|
51.20 |
- |
| 21 |
Parrish, Isabella M (12)
|
MRSSC |
54.72 |
52.65 |
-2.07 |
| 22 |
Ali, Katina L (11)
|
MRWISC |
45.99 |
53.22 |
7.23 |
| 23 |
Morda, Amanda M (11)
|
MRSSC |
55.49 |
53.83 |
-1.66 |
| 24 |
Ratto, Leah F (12)
|
MRSSC |
55.59 |
54.98 |
-0.61 |
| 25 |
Buffolino, Angelina (11)
|
MRTS |
1:04.95 |
55.32 |
-9.63 |
| 26 |
Moran, Grace E (12)
|
MRLBA |
|
57.79 |
- |
| 27 |
Meglino, Samantha M (12)
|
MRSSC |
1:15.59 |
1:05.10 |
-10.49 |
|
Mahady, Madison G (11)
|
MRSSC |
1:05.00 |
DQ |
- |
|
Hillyard, Casey A (11)
|
MRSSC |
1:02.74 |
DQ |
- |
|
Stewart, Jordan M (11)
|
MRLBA |
|
DQ |
- |